Nitrocellulose Membranes
High quality nitrocellulose membranes in convenient formats for Western blotting and other immunoblotting techniques
High quality nitrocellulose membranes in convenient formats for Western blotting and other immunoblotting techniques:
- Pure nitrocellulose is the most frequently used membrane for the transfer of proteins and is compatible with a wide range of detection protocols
- 100% nitrocellulose with a smooth surface finish and a bright, white color
- Proven medium for Western blotting that produces excellent signal-to-noise results
- BSA protein binding capacity of 160 µg/cm2-can bind a wide range of fragment sizes and can still be easily blocked for low background
- High wick rates (lateral flow)-substances move quickly through the membrane
Applications
- Transfer of low molecular–weight proteins and nucleic acids <500 bp
- Protein transfer from SDS-PAGE gels to membrane in preparation for Western blotting
- Transfer of nucleic acids in preparation for northern or Southern blotting
- Immunodetection of target protein and probe hybridization of nucleic acids
- Non-specific binding can be blocked with BSA, gelatin, caesin, or other surface passivating agents